Study of the improved Sf9 transient gene expression process
نویسندگان
چکیده
Introduction Insect cells have been widely used for the production of recombinant proteins using recombinant baculovirus for gene delivery [1]. To simplify protein production in insect cells, we have previously described a method, based on transient gene expression (TGE) with cultures of suspension-adapted Sf9 cells using polyethylenimine (PEI) for DNA delivery [2]. Expression of GFP has been realized at high efficiency and a tumor necrosis factor receptor-Fc fusion protein (TNFR-Fc) was produced at a level of 40 mg/L. However, the efficiency of the insect cells TGE system has not been studied and further optimization may improve protein titers. Here, we studied the efficiency of PEI for plasmid delivery in Sf9 cells.
منابع مشابه
Transient Expression of Foot and Mouth Disease Virus (FMDV) Coat Protein in Tobacco (Nicotiana tabacom) via Agroinfiltration
Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants. Objective: The main goal of the present study was to eva...
متن کاملExpression of Recombinant Factor IX Using the Transient Gene Expression Technique
Background: Pilot and large-scale production of recombinant proteins requires the presence of stable clones capable of producing large quantities of recombinant proteins. Not only the process of selecting stable clones is time consuming, but also the continuous culturing of clones in large-scale production may cause loss of incoming plasmid and recombinant genes. Thus, considering the advanceme...
متن کاملHuman Papillomavirus Type16- L1 VLP Production in Insect Cells
Objective(s): Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (VLP). VLPS are non-infectious, highly immunogenic and can elicit neutralizing...
متن کاملProviding a mathematical model for measuring the expression of GUS gene was transferred temporarily through xylem vessels using RT-PCR and probe Gold nanoparticles
Gene transfer to plants and the production of transgenic plants with various purposes, such as improving the performance and quality, resistance to pests, diseases, etc., and of great importance are carried out Gene transfer to plants performs to evaluate the transient and permanent gene expression. Transient expression is quick, easy and simple and is not influenced by position effect compare ...
متن کاملProviding a mathematical model for measuring the expression of GUS gene was transferred temporarily through xylem vessels using RT-PCR and probe Gold nanoparticles
Gene transfer to plants and the production of transgenic plants with various purposes, such as improving the performance and quality, resistance to pests, diseases, etc., and of great importance are carried out Gene transfer to plants performs to evaluate the transient and permanent gene expression. Transient expression is quick, easy and simple and is not influenced by position effect compare ...
متن کامل